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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
JoVE Journal
Biology
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JoVE Journal Biology
Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

10,160 Views

07:55 min

June 26, 2020

DOI:

07:55 min
June 26, 2020

10141 Views
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Summary

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Following DNA damage, human cells activate essential repair pathways to restore the integrity of their genome. Here, we describe the method of indirect immunofluorescence as a means to detect DNA repair proteins, analyze their spatial and temporal recruitment, and help interrogate protein-protein interaction at the sites of DNA damage.

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