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Journal / Immunology and Infection / Analyse av human naturlig killer celle metabolisme
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse av human naturlig killer celle metabolisme
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Analyse av human naturlig killer celle metabolisme

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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I dette papiret beskriver vi en metode for å måle glykose og mitokondriegånding i primære humane Natural Killer (NK) celler isolert fra perifert blod, i ro eller etter IL15-indusert aktivering. Protokollen som beskrives kan lett utvides til primære menneskelige NK-celler aktivert av andre cytokiner eller løselige stimuli.

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Immunologi og infeksjon utgave 160 Naturlige Killer celler metabolisme ekstracellulær fluks glycolyse mitokondrier åndedrett elektron transportkjede strømningscytometri
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