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Journal / Cancer Research / 从Sézary综合征患者中分离人外周血单核细胞和CD4 +…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
从Sézary综合征患者中分离人外周血单核细胞和CD4 + T细胞以进行转录组学分析
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从Sézary综合征患者中分离人外周血单核细胞和CD4 + T细胞以进行转录组学分析

Article DOI: 10.3791/61470-v • 09:08 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Department of Dermatology, University of Arkansas for Medical Sciences

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我们提出了一种简单的方案，用于从诊断为Sézary综合征的患者获得的全血中分离外周血单核细胞，然后选择CD4 + T细胞，用phorbol12-肉豆蔻酸酯13-乙酸盐和A23187离子载体刺激它们，并制备RNA进行转录组学分析。

Tags

癌症研究，第176期，皮肤T细胞淋巴瘤，Sézary综合征，外周血单核细胞，佛波bol-12-肉豆蔻酸酯13-乙酸酯，A23187，微阵列，进展，肿瘤。
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