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JoVE Journal Cancer Research
Aislamiento de células mononucleares de sangre periférica humana y células T CD4 + de pacientes con síndrome de Sézary para perfiles transcriptómicos
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Aislamiento de células mononucleares de sangre periférica humana y células T CD4 + de pacientes con síndrome de Sézary para perfiles transcriptómicos

Article DOI: 10.3791/61470-v • 09:08 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Department of Dermatology, University of Arkansas for Medical Sciences

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Presentamos un protocolo sencillo para el aislamiento de células mononucleares de sangre periférica a partir de sangre total obtenida de pacientes diagnosticados con Síndrome de Sézary, seguido de selección de linfocitos T CD4+, su estimulación con forbol12-miristato13-acetato e ionóforo A23187, y preparación de ARN para el perfil transcriptómico.

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Cancer Research Número 176 linfoma cutáneo de células T síndrome de Sézary célula mononuclear de sangre periférica forbol-12-mirisato13-acetato A23187 microarray progresión neoplasia.
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