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Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
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JoVE Journal Bioengineering
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

DOI:
10.3791/61496-v

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07:50 min

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June 02, 2020

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, ,
1Department of Chemical and Environmental Engineering,University of Cincinnati, 2Department of Biomedical Engineering,University of Cincinnati, 3Neuroscience Graduate Program,University of Cincinnati College of Medicine

Chapters

  • 00:04Introduction
  • 00:55Tunable Cell Culture Substrate Preparation
  • 05:34Results: Dynamics of Schwann Cell Phenotype on PDMS Substrates
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

This methodology aims to illustrate the mechanisms by which extracellular matrix cues such as substrate stiffness, protein composition and cell morphology regulate Schwann cell (SC) phenotype.

Tags

Keywords: Extracellular MatrixMicroenvironmentSchwann CellCell PhenotypePDMSMicrocontact PrintingSubstrate StiffnessProtein CompositionCell MorphologyNervous System RegenerationCell Culture Platform

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