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Accessing the Cytotoxicity and Cell Response to Biomaterials
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Accessing the Cytotoxicity and Cell Response to Biomaterials

Article DOI: 10.3791/61512-v • 09:46 min • July 8th, 2021
July 8th, 2021 •
*1,2,3,4, *2,3,4,5, 1,2,3,4, 2,3,4,5, 2,3,4,6, 2,3,4,6, 2,3,4,7, 2,3,4,5, 1,2,3,4,8, 1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
* These authors contributed equally

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Summary
Automatic Translation

This methodology aims to evaluate biomaterial cytotoxicity through the preparation of soluble extracts, using viability assays and phenotypic analysis, including flow cytometry, RT-PCR, immunocytochemistry, and other cellular and molecular biology techniques.

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Cytotoxicity Cell Response Biomaterials Medical Applications Screening Researchers Novel Biomaterials Quantitative Evaluation Qualitative Evaluation Bioactivity Pharmaceutical Formulations Cell Exposure Assays Clinical Practice Appropriate Biomaterial Dentistry Orthopedics Surgery Ophthalmology Cardiology Characterization Living Tissues PVC Molds Sterilize Pellets
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