May-grunwald Giemsa
May-grunwald Giemsa - Related Videos
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
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2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
02:52
Giemsa Staining for Colony Formation Assay: An In Vitro Staining Procedure to Assess Proliferative Capability of Mesenchymal Stem Cells
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2025
In this video, we demonstrate the Giemsa staining procedure to assess the proliferative potential of mesenchymal stem cells to form viable colonies. Giemsa stain is a neutral stain primarily composed of azure and eosin dyes, which carry selective affinity for cellular components depending on their charge.
10:21
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Cited by 3 •
2018
Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.
08:00
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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Cited by 29 •
2016
This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.
03:56
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
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2023
This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic biomarker.
FAQs
The method combines acidic eosin with basic azure and methylene blue dyes. Cellular components acquire different colors according to their chemical properties, allowing nuclei, cytoplasm, and granules to appear with contrasting visual characteristics. This chemical differentiation is what enables microscopic examination to distinguish blood-cell structures rather than displaying the smear as a uniformly colored field.
Methanol fixation is a preparatory step used before the staining sequence, particularly for blood and bone marrow smears. Its inclusion establishes the condition in which the dye mixture interacts with the cellular material. Because the final appearance depends on staining conditions, consistent preparation is important when comparing nuclear, cytoplasmic, and granule detail between specimens.
Staining conditions influence how clearly nuclear, cytoplasmic, and granule features are visualized. Changes in those conditions can therefore affect the contrast used to distinguish leukocyte types and assess cell morphology. In hematology, controlling the staining process matters because poorly differentiated features may make abnormal or immature populations more difficult to recognize.
These cellular regions provide different morphological information and respond within the stain's chemically differentiated color pattern. Examining them separately helps reveal the characteristics used to identify leukocyte types and evaluate whether cells appear normal, abnormal, or immature. The resulting detail supports interpretation of both individual cell morphology and the broader cellular composition of a specimen.
A typical workflow begins with a peripheral blood or bone marrow smear, followed by methanol fixation and application of the combined acidic and basic dyes. The prepared smear is then examined microscopically for cellular detail. This sequence produces the visual contrast needed to assess leukocyte types, morphology, and the presence of abnormal or immature populations.
The procedure requires a blood or bone marrow smear, methanol for the usual fixation step, the acidic eosin and basic azure-methylene blue dye components, and microscopy for evaluation. Each contributes a different part of the workflow: the smear supplies the cellular material, fixation precedes staining, the dyes create contrast, and microscopy reveals diagnostic morphology.
It is especially useful when investigators or diagnosticians need to characterize cells in peripheral blood or bone marrow. The resulting morphology can help identify leukocyte types, assess abnormal or immature populations, and support disease classification. Beyond routine diagnosis, the method also contributes to research involving blood and hematopoietic disorders by making cellular patterns directly observable.