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Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter
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Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter

Article DOI: 10.3791/61517-v • 08:33 min • August 5th, 2020
August 5th, 2020 •
1,2, 1,2, 1, 1,2,3, 1,2,3, 1,3
1Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

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Præsenteret her er en protokol for laser-capture microdissection (LCM) af plantevæv. LCM er en mikroskopisk teknik til isolering af vævsområder på en forureningsfri måde. Proceduren omfatter vævsfiksering, paraffinindlejring, sektionsindtrækning, LCM- og RNA-ekstraktion. RNA anvendes i den downstream-vævsspecifikke, tidsmæssigt løste analyse af transskriptomer.

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Biologi laser-capture mikrodissektion LCM transskription vævsspecifik RNA-seq genekspression rumlige
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