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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Article DOI: 10.3791/61575-v • 13:25 min • May 15th, 2021
May 15th, 2021 •
*1, *1, *1,2, 1, 1, 1, 1,2
1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

Tags

Acute Kidney Injury Cisplatin Adult Zebrafish Nephrotoxic Agent Inflammation Cell Death Kidney Tissue Anesthetized Specific Dose Peritoneal Cavity Monitor Mortality Dissected Kidney Flow Cytometry Fluorescent TUNEL Assay Renal Field Therapeutic Targets Renal Protection Mechanism Of Regeneration Chemotherapy Agent Cancer Treatment Accumulation In Kidney Decrease Kidney Function Dose Dependent Inflammation Analysis Cell Death Analysis Disease Model
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