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Journal / Biochemistry / 通过 EFA 去卷和散射分析 SEC-SAXS 数据
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Biochemistry

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JoVE Journal Biochemistry
通过 EFA 去卷和散射分析 SEC-SAXS 数据
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通过 EFA 去卷和散射分析 SEC-SAXS 数据

Article DOI: 10.3791/61578-v • 10:59 min • January 28th, 2021
January 28th, 2021 •
*1, 2, 3, *4
1European Synchrotron Radiation Facility Structural Biology Group, Structural Biology Group, 2Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 3Diamond Light Source, 4Laboratoire de Physiologie Cellulaire and Végétale, Université Grenoble Alpes/CNRS/CEA/INRA/BIG
* These authors contributed equally

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Summary
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SEC-BioSAXS 生物大分子的测量是确定大分子及其复合物溶液结构的标准方法。在这里，我们分析来自两种常见 SEC 跟踪的 SEC-BioSAXS 数据 - 具有完全解析和部分解析峰值的色谱图。我们使用散射和 BioXTAS RAW 演示分析与去卷。

Tags

生物化学， 问题 167， 生物小角度 X 射线散射， BioSAXS， 内联大小排除色谱， SEC， 背景减法， E9， vaccinia， 散射， SEC-SAXS 数据去卷， BioXTAS RAW 内联大小排除色谱与生物小角度 X 射线散射 （SEC-BioSAX） 耦合
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