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Journal
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Biology
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异种移植模型中循环猪特异性DNA的定量
JoVE Journal
Biology
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JoVE Journal
Biology
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
Please note that all translations are automatically generated.
Click here for the English version.
异种移植模型中循环猪特异性DNA的定量
DOI:
10.3791/61579-v
•
07:34 min
•
September 22, 2020
•
Yangyang Deng*
1,2
,
Ming Zhou*
1,3
,
Ying Lu
,
Jiao Chen
,
Zuhui Pu
,
Dongjing Yu
5
,
Yifan Dai
,
Yongqiang Zhan
,
Lisha Mou
1
Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center
,
Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital
,
2
Xianning Hospital of Traditional Chinese Medicine
,
3
Liver-biotechnology (Shenzhen) Co., Ltd.
,
4
Department of Radiology
,
Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital
,
5
Department of Life Sciences
,
University of Toronto
,
6
Jiangsu Key Laboratory of Xenotransplantation
,
Nanjing Medical University
,
7
Department of Hepatopancreatobiliary Surgery
,
Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital
Chapters
00:04
Introduction
00:52
Isolating Genomic DNA
02:20
Verification of Primer Specificity
03:42
Standard Curve of cpsDNA
04:45
Quantitation of Circulating Pig-specific DNA
05:55
Results: PCR Validation of Porcine Specific Primers
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在该协议中,设计了猪特异性底剂,构建了含质粒的猪特异性DNA片段,并建立了定量标准曲线。使用物种特异性底法,cpsDNA在猪对小鼠细胞移植模型和猪对猴子动脉贴片移植模型中通过qPCR进行量化。
Tags
Xenotransplantation
Pig-specific DNA
Blood
Monitoring
Immune Rejection
PCR
DNA Extraction
Agarose Gel Electrophoresis
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