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异种移植模型中循环猪特异性DNA的定量
JoVE Journal
Biology
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JoVE Journal Biology
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
DOI:

07:34 min

September 22, 2020

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Chapters

  • 00:04Introduction
  • 00:52Isolating Genomic DNA
  • 02:20Verification of Primer Specificity
  • 03:42Standard Curve of cpsDNA
  • 04:45Quantitation of Circulating Pig-specific DNA
  • 05:55Results: PCR Validation of Porcine Specific Primers
  • 06:51Conclusion

Summary

Automatic Translation

在该协议中,设计了猪特异性底剂,构建了含质粒的猪特异性DNA片段,并建立了定量标准曲线。使用物种特异性底法,cpsDNA在猪对小鼠细胞移植模型和猪对猴子动脉贴片移植模型中通过qPCR进行量化。

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