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硅纳米线与光学刺激研究细胞内和细胞间电耦合
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling
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硅纳米线与光学刺激研究细胞内和细胞间电耦合

DOI:
10.3791/61581-v

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08:58 min

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January 28, 2021

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, , ,
1The James Franck Institute,The University of Chicago, 2Department of Chemistry,The University of Chicago, 3The Institute for Biophysical Dynamics,The University of Chicago

Chapters

  • 00:04Introduction
  • 00:45Cell-Silicon Nanowire (SiNWs) Hybrid Preparation
  • 04:02Cardiomyocyte (CM):Myofibroblast SiNW Hybrid Co-Culture
  • 05:07Optical Imaging and Stimulation
  • 06:35Results: Representative Intra- and Intercellular Electrical Calcium Coupling Analyses
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了使用硅纳米线在细胞内光学生物调制细胞在简单和易于执行的方法。该技术高度适应不同的细胞类型，可用于体外和体内应用。

Tags

Silicon NanowiresOptical StimulationIntracellular Bioelectrical InterrogationCell SpecificityCardiac CellsBrain CellsElectrical CommunicationMyofibroblastsCardiomyocytesChemical Vapor DepositionNanowire SuspensionCollagen Coating

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