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Journal / Bioengineering / Manipulação de células-tronco neurais únicas e…
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Bioengineering

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JoVE Journal Bioengineering
Manipulação de células-tronco neurais únicas e neurônios em fatias cerebrais usando microinjeção robótica
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Manipulação de células-tronco neurais únicas e neurônios em fatias cerebrais usando microinjeção robótica

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Este protocolo demonstra o uso de uma plataforma robótica para microinjeção em células-tronco neurais únicas e neurônios em fatias cerebrais. Esta técnica é versátil e oferece um método de rastreamento de células em tecido com alta resolução espacial.

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Bioengenharia Edição 167 desenvolvimento cerebral células-tronco rastreamento de linhagem robótica neurônio microinjeção fatia de tecido organotípico manipulação celular única
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