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Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
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Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Article DOI: 10.3791/61605-v • 07:02 min • November 3rd, 2020
November 3rd, 2020 •
1,2, 1,2, 3, 1,2, 1,2
1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter, University Rostock, 3Department of Cardiology, Rostock University Medical Center

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The formation of a proper sarcomere network is important for the maturation of iPSC-derived cardiomyocytes. We present a super resolution-based approach that allows for the quantitative evaluation of the structural maturation of stem cell derived cardiomyocytes, to improve culture conditions promoting cardiac development.

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α-Actinin Network Human IPSC-Derived Cardiomyocytes Single Molecule Localization Microscopy Quantitative Evaluation Sarcomere Maturation Super Resolution-based Approach Confocal Imaging PALM Imaging Buffer Microscope Stage Holder 1.57 NA 100X Oil Objective PALM Mode TIRF Settings UV Laser Power Gain Level Acquisition Parameters Target Cell PALM Image Acquisition Reconstruction Of PALM Data ImageJ Thunderstorm Plugin
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