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A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

DOI:
10.3791/61606-v

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08:40 min

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August 07, 2020

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, , , , ,
1Department of Medical Cell Biology,Uppsala University, 2Polymer Chemistry, Department of Chemistry-Ångström Laboratory,Uppsala University

Chapters

  • 00:04Introduction
  • 00:51Fibrinogen Stock Solution
  • 01:47Insert Coating
  • 03:12Liver Cell Seeding
  • 05:40Endothelial Cell Seeding
  • 06:44Results: Representative Biomimetic Liver Cancer Model Preparations and Analyses
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.

Tags

3D Tumor ModelBiomimeticTumor-stroma InteractionsLiver CancerHydrogelCell CultureCollagen CoatingFibrinogenCell LinesHepatocellular CarcinomaMicroenvironment

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