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Cancer Research

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JoVE Journal Cancer Research
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
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A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

Article DOI: 10.3791/61606-v • 08:40 min • August 7th, 2020
August 7th, 2020 •
1, 1, 2, 1, 2, 1
1Department of Medical Cell Biology, Uppsala University, 2Polymer Chemistry, Department of Chemistry-Ångström Laboratory, Uppsala University

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This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.

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Biomimetic Model Liver Cancer Tumor-stroma Interactions 3D Environment Bio-physical Properties Hydrogels Stiffness Hepatocellular Cell Lines Stroma Associated Cell Lines Cost-effective Basic Equipment Complex Tumor-stroma Interactions Hepatocarcinogenesis Mechanistic Perspective Treatment Perspective Carlemi Calitz Jenny Rosenquist Fibrinogen Stock Solution Calcium Chloride Aprotinin Distilled Water PBS Sodium Chloride Shaker
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