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Real-Time Monitoring van Aurora kinase A Activatie met behulp van Conformational FRET Biosensors in Live Cells
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Real-Time Monitoring van Aurora kinase A Activatie met behulp van Conformational FRET Biosensors in Live Cells

Article DOI: 10.3791/61611-v • 06:29 min • July 30th, 2020
July 30th, 2020 •
1, 1, 1
1Univ Rennes, CNRS, IGDR (Genetics and Development Institute of Rennes), UMR 6290, F-35000 Rennes, France

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De activering van de multifunctionele Ser/Thr kinase AURKA wordt gekenmerkt door zijn autofosforylering op Thr288. Fluorescerende sondes die op FRET vertrouwen, kunnen onderscheid maken tussen de inactieve en geactiveerde toestanden. Hier illustreren we enkele strategieën voor sonde-engineering, samen met een snel FRET-protocol om de kinase-activering tijdens mitose te volgen.

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Biologie AURKA FRET FLIM genetisch gecodeerde biosensoren conformatieverandering activering mitose levende cellen
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