Journal
/
Immunology and Infection
/
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

DOI:
10.3791/61631-v

•

07:25 min

•

July 16, 2020

•

, , , , ,
1Translational Medicine,Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics,University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Summary

Automatic Translation

Automatic Translation

This protocol provides methods for visualization of bacterial cells and polysaccharide synthesis locus (Psl) polysaccharide within the sputum of cystic fibrosis patients.

Tags

Keywords: Pseudomonas AeruginosaCystic FibrosisSputumVisualizationHydrogelExopolysaccharidePslBiofilmAntimicrobial SusceptibilityFixationAcrylamideAnaerobicSDSClearing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61631?article_type=v

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
check_url/61631?article_type=v

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
check_url/61631?article_type=v

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
check_url/61631?article_type=v

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
check_url/61631?article_type=v

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
check_url/61631?article_type=v

Pseudomonas aeruginosa Induced Lung Injury Model
check_url/61631?article_type=v

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways
check_url/61631?article_type=v

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
check_url/61631?article_type=v

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
check_url/61631?article_type=v

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Read Article