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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering av Pseudomonas aeruginosa i sputum hos patienter med cystisk fibros
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Visualisering av Pseudomonas aeruginosa i sputum hos patienter med cystisk fibros

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Detta protokoll tillhandahåller metoder för visualisering av bakterieceller och polysackaridsynteslokus (Psl) polysackarid i sputum hos patienter med cystisk fibros.

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Immunologi och infektion utgåva 161 Kronisk infektion Lungfunktion sjuklighet Antibiotikabehandling Pediatriska patienter Rumslig organisation Aggregering Biofilmbildning Polysackaridproduktion Sputumprover Vävnadsrensningsteknik Hydrogelmatris 3D-strukturer Fluorescerande etiketter Fluorescens In Situ-hybridisering Anti-Psl-antikroppar Exopolysackarid DAPI-färgning värdceller högupplöst avbildning
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