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Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

DOI:
10.3791/61635-v

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10:41 min

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September 12, 2020

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, , ,
1Department of Neurology,University Hospital of Würzburg

Chapters

  • 00:00Introduction
  • 00:47Priming of Osmotic Pumps
  • 01:46Implantation of Brain Cannulas and Osmotic Pumps
  • 05:50Stress Paradigm
  • 07:39Characterization of the Phenotype: Rating Scales
  • 09:31Results
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a protocol to generate a pharmacological DYT/PARK-ATP1A3 dystonia mouse model via implantation of cannulas into basal ganglia and cerebellum connected to osmotic pumps. We describe the induction of dystonia-like movements via application of a motor challenge and the characterization of the phenotype via behavioral scoring systems.

Tags

Keywords: Osmotic PumpsBrain CannulasPharmacological Mouse ModelDYT/PARK-ATP1A3 DystoniaMotor StressGene-environment InteractionsMovement DisordersStereotactic SurgeryBasal GangliaCerebellum

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