7,443 Views
•
10:10 min
•
August 19, 2020
DOI:
Summary
Automatically generated
Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.
Related Videos
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Related Videos
9465 Views
08:53
Localization, Identification, and Excision of Murine Adipose Depots
Related Videos
41057 Views
09:04
A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
Related Videos
9271 Views
12:05
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
Related Videos
9225 Views
08:52
Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue
Related Videos
27168 Views
06:19
Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
Related Videos
16477 Views
06:31
Identification and Dissection of Diverse Mouse Adipose Depots
Related Videos
40505 Views
06:35
Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue
Related Videos
934 Views
09:08
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Related Videos
6156 Views
04:46
Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
Related Videos
1363 Views
Read Article
Cite this Article
Gilleron, J., Meziat, C., Sulen, A., Ivanov, S., Jager, J., Estève, D., Muller, C., Tanti, J., Cormont, M. Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging. J. Vis. Exp. (162), e61640, doi:10.3791/61640 (2020).
Copy