Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.
Gilleron, J., Meziat, C., Sulen, A., Ivanov, S., Jager, J., Estève, D., Muller, C., Tanti, J., Cormont, M. Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging. J. Vis. Exp. (162), e61640, doi:10.3791/61640 (2020).