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Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
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Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

DOI:
10.3791/61651-v

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07:45 min

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September 10, 2020

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,
1Department of Entomology,The Ohio State University, 2Insect Transformation Facility, Institute for Bioscience and Biotechnology Research,University of Maryland

Chapters

  • 00:04Introduction
  • 01:10Injecting Culex Embryos
  • 05:44Results: Transmission of the Desired Mutation
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

CRISPR/Cas9 is increasingly used to characterize gene function in non-model organisms. This protocol describes how to generate knock-out lines of Culex pipiens, from preparing injection mixes, to obtaining and injecting mosquito embryos, as well as how to rear, cross, and screen injected mosquitoes and their progeny for desired mutations.

Tags

CRISPR/Cas9Mosquito EmbryoMicro-injectionCulex MosquitoesWest Nile FeverSt. Louis EncephalitisFilarial NematodesCanine HeartwormElephantiasisGenome EditingMosquito SurvivalNull Mutations

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