Here, we present a protocol for preparing and culturing a blood brain barrier metastatic tumor micro-environment and then quantifying its state using confocal imaging and artificial intelligence (machine learning).
Oliver, C. R., Westerhof, T. M., Castro, M. G., Merajver, S. D. Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography. J. Vis. Exp. (162), e61654, doi:10.3791/61654 (2020).