Endothelial Barrier
Endothelial Barrier - Related Videos
09:23
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
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Cited by 31 •
2017
The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
12:30
Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
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Cited by 213 •
2014
This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.
10:17
An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
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Cited by 12 •
2016
Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.
09:36
Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
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Cited by 59 •
2012
This method describes how to isolate and immortalize microvascular endothelial cells from mouse brain. We describe a step-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding and immortalization of the cells. Usually, it takes about five weeks to obtain a homogenous, immortalized microvascular endothelial cell line.
07:19
Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
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Cited by 18 •
2013
In vitro traumatic brain injury models are being developed to reproduce in vivo brain deformation. Stretch-induced injury has been employed for astrocytes, neurons, glial cells, aortic, and brain endothelial cells. However, our system uses a blood brain barrier (BBB) model that possesses properties constituting a legitimate model of the BBB to establish an in vitro TBI model.
FAQs
Tight and adherens junctions restrict movement between neighboring endothelial cells, while the cytoskeleton helps maintain and adjust cell structure. The basement membrane provides an additional supporting layer beneath the cells. Together, these components determine how readily fluids, solutes, immune cells, and macromolecules cross vessel walls, making their coordinated behavior central to barrier stability.
Permeability changes when endothelial cells respond to signaling molecules, inflammation, mechanical forces, or tissue injury. These influences can alter the relationships among cell junctions, the cytoskeleton, and the basement membrane, changing exchange across the vessel wall. Examining these triggers helps explain why vascular leakage occurs under different biological conditions.
Barrier regulation determines whether fluids and macromolecules remain within vessels and whether immune cells can move into surrounding tissue. During inflammation or injury, altered permeability can promote vascular leakage and edema, while changes in cellular interactions can support leukocyte migration. These outcomes connect endothelial behavior with the progression and study of tissue responses.
A useful investigation considers permeability together with the condition of tight and adherens junctions, the endothelial cytoskeleton, and the basement membrane. Researchers can then relate changes in exchange across vessel walls to signaling molecules, inflammation, mechanical forces, or injury. This combined view helps distinguish structural regulation from the resulting biological outcomes, such as leakage or immune-cell passage.
Studies of the barrier connect vessel-wall regulation with vascular leakage, edema, and inflammatory cell migration, all of which provide relevant context for cardiovascular disease research. By examining how junctions and supporting structures respond to damaging or inflammatory conditions, investigators can analyze changes in vascular exchange and identify biological processes associated with impaired barrier function.
Blood-brain barrier function depends on regulated endothelial exchange, so barrier studies help clarify how solutes and macromolecules may be controlled at this specialized vascular interface. The same principles inform drug-delivery research by identifying how permeability changes could affect transport. Endothelial barrier knowledge also supports tissue-engineering studies that aim to reproduce controlled vessel-wall behavior.