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Biochemistry
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使用免疫增强技术的超高速西布洛特
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
Please note that all translations are automatically generated.
Click here for the English version.
使用免疫增强技术的超高速西布洛特
DOI:
10.3791/61657-v
•
05:59 min
•
September 26, 2020
•
Sayuri L. Higashi
2
,
Kazuya Yagyu
2
,
Haruna Nagase
2
,
Craig S. Pearson
,
Herbert M. Geller
,
Yasuhiro Katagiri
1
Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute
,
National Institutes of Health
,
2
United Graduate School of Drug Discovery and Medical Information Sciences
,
Gifu University
Chapters
00:05
Introduction
00:51
Incubation with Primary Antibody
01:59
Membrane Wash
02:59
Image Acquisition
03:52
Results: Optimization and Validation of CDR Method in conjunction with IRE
05:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
通过循环排泄和补充 (CDR) 技术与免疫增异剂一起改进抗原抗体结合的动能,开发出一种超高速西方印迹技术。
Tags
Ultra-high-speed Western Blot
Immunoreaction Enhancing Technology
PVDF Membrane
Primary Antibody
Secondary Antibody
Chemiluminescent Substrate
Salad Spinner
Cyclic Draining And Replenishing
Biomedical Research
Time-saving
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