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JoVE Journal
Neuroscience

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Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
 

Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse

Article DOI: 10.3791/61659-v 09:39 min August 26th, 2020
August 26th, 2020

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Summary

In vivo microendoscopic calcium imaging is an invaluable tool that enables real-time monitoring of neuronal activities in freely behaving animals. However, applying this technique to the amygdala has been difficult. This protocol aims to provide a useful guideline for successfully targeting amygdala cells with a miniaturized microscope in mice.

Tags

In Vivo Calcium Imaging Miniaturized Microscopy Amygdala Genetically Defined Cell Populations AAV Injection Craniotomy Dorsoventral Coordinate Lens Implantation Viral Genomes Per Milliliter Pipette Injection Site
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