Journal
/
Biology
/
Bovine Ovarian Cortex Tissue Culture
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Bovine Ovarian Cortex Tissue Culture

Bovine Ovarian Cortex Tissue Culture

DOI:
10.3791/61668-v

•

06:32 min

•

January 14, 2021

•

, , ,
1Department of Animal Science,University of Nebraska-Lincoln, 2Department of Animal Production, School of Agriculture,University of Jordan

Chapters

  • 00:05Introduction
  • 01:07Ovarian Cortical Culture Protocol
  • 03:15Media Collection, Imaging, and Downstream Processing
  • 04:39Results: Analysis of H & E Staining, Number of Follicles at Different Stages, and Collagen Staining of the Ovarian Cortex
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro culture of bovine ovarian cortex and the effect of nutritional Stair-step diet on ovarian microenvironment is presented. Ovarian cortex pieces were cultured for seven days and steroids, cytokines, and follicle stages were evaluated. The Stair-Step diet treatment had increased steroidogenesis resulting in follicle progression in culture.

Tags

Keywords: Bovine Ovarian CortexFollicle GrowthSteroidogenesisImmune MoleculesReproductive DisordersPolycystic Ovarian SyndromeOvarian MicroenvironmentIn Vitro Examination

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61668?article_type=v

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
check_url/61668?article_type=v

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
check_url/61668?article_type=v

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
check_url/61668?article_type=v

Bovine Mammary Gland Biopsy Techniques
check_url/61668?article_type=v

Reproductive Techniques for Ovarian Monitoring and Control in Amphibians
check_url/61668?article_type=v

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
check_url/61668?article_type=v

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture
check_url/61668?article_type=v

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
check_url/61668?article_type=v

Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)
check_url/61668?article_type=v

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Read Article