Ovarian Microenvironment
Ovarian Microenvironment - Related Videos
10:35
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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Cited by 18 •
2010
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
09:04
Probing the Ovarian Microenvironment: Methods for Isolation, 2D Culture, and Organoid Culture of Mouse Ovarian Somatic Cells
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2026
We describe methods for isolating mouse ovarian somatic cells from a stroma-enriched fraction of the ovary and for generating mouse ovarian somatic organoids using a scaffold-free approach.
09:00
Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity
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Cited by 20 •
2015
Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.
11:57
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
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Cited by 25 •
2012
A combinatorial functional screening method for gaining insights into the impacts of the molecular composition of microenvironments on cellular functions is described. The method takes advantage of existing microarray-based technologies to generate arrays of defined combinatorial microenvironments that support cell adhesion and functional analysis.
FAQs
The extracellular matrix provides a local framework that surrounds follicles and oocytes while participating in communication with nearby cells and signaling molecules. Its relationship with granulosa, theca, stromal, and immune cells helps shape the conditions in which follicles develop and function. This makes the matrix relevant to understanding how local tissue organization affects reproductive biology.
Oocytes, granulosa cells, theca cells, stromal cells, immune cells, and vascular tissues communicate within a shared local environment. Their coordinated signaling supports follicle growth and steroid production rather than allowing each cell type to act independently. Examining these interactions helps explain how cellular cooperation contributes to normal ovarian function and reproductive outcomes.
Blood vessels and hormones help connect local ovarian activity with the resources and signals needed for follicle function. Vascular tissues contribute to nutrient exchange, while hormonal and molecular signals participate in regulating development and steroid production. Together, these influences help create changing conditions around follicles and oocytes as ovarian processes proceed.
Alterations in the local cellular, extracellular, vascular, hormonal, or signaling environment can change the conditions surrounding follicles and oocytes. Such changes may influence oocyte quality and are associated with reproductive aging, infertility, or ovarian disease. Studying these relationships helps connect local ovarian biology with broader changes in reproductive function.
Researchers examine the interactions among follicles, oocytes, surrounding cell populations, extracellular matrix, blood vessels, hormones, and signaling molecules. They consider how these components influence follicle growth, steroid production, nutrient exchange, ovulation, and oocyte quality. This integrated focus is important because ovarian outcomes arise from communication across the local environment rather than from one component alone.
Understanding the local conditions surrounding follicles and oocytes can help researchers identify factors associated with development, function, and oocyte quality. That knowledge supports efforts to improve fertility treatments by considering cellular communication, nutrient exchange, hormonal signals, and tissue context. The approach therefore extends beyond studying the oocyte in isolation and emphasizes its surrounding biological environment.
The ovarian microenvironment provides a biological framework for modeling how cells, matrix, vessels, hormones, and signaling molecules interact during ovarian function and disease. Reproducing or studying these relationships can support disease models and tissue-engineering strategies. Such work may help researchers investigate ovarian disorders while developing systems that more closely reflect the conditions surrounding follicles and oocytes.