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Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants’ Extracts and Fractions to Prevent Dental Caries
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JoVE Journal Biology
Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants’ Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants’ Extracts and Fractions to Prevent Dental Caries

DOI:
10.3791/61773-v

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08:20 min

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March 31, 2021

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, , , , ,
1Department of Dental Materials and Prosthodontics, School of Dentistry,São Paulo State University (UNESP), 2Department of Organic Chemistry, Institute of Chemistry,São Paulo State University (UNESP), 3Faculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP), Department of Physics and Chemistry,University of São Paulo

Chapters

  • 00:04Introduction
  • 00:53Preparation of Crude Extracts (CE) and Fractions (CEF) to Chemical Profile Analysis and Bioassays
  • 02:26Bioassay and Anti-microbial Activity
  • 04:10Validation of Biological Activity and Biological Data Analysis
  • 06:16Results: The Screening Results of the Twelve Crude Extracts from Three Varieties of C. sylvestris Against S. mutans
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

Natural products represent promising starting points for the development of new drugs and therapeutic agents. However, due to the high chemical diversity, finding new therapeutic compounds from plants is a challenging and time-consuming task. We describe a simplified approach to identify antimicrobial and antibiofilm molecules from plant extracts and fractions.

Tags

Keywords: AntimicrobialAntibiofilmNatural ProductsDental CariesBiofilmPlant ExtractsFractionationS. MutansAnti-microbial AssayBiofilm Assay

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