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Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
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Cancer Research
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JoVE Journal Cancer Research
Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

DOI:
10.3791/61775-v

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07:03 min

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October 02, 2020

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, , , ,
1London Regional Cancer Program, 2Department of Anatomy & Cell Biology,Western University, 3Department of Oncology,Western University, 4Lawson Health Research Institute

Chapters

  • 00:05Introduction
  • 00:38Breast Cancer Cell Culture Single Cell Suspension Generation
  • 01:22Tissue Sample Single Cell Suspension Generation
  • 02:45Colony Forming Assay
  • 03:54Mammosphere Assay and 3D Culture Model
  • 05:09Results: Representative BCSC Identification and Functional Evaluation
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

This experimental protocol describes the isolation of BCSCs from breast cancer cell and tissue samples as well as the in vitro and in vivo assays that can be used to assess BCSC phenotype and function.

Tags

Keywords: Breast Cancer Stem CellsCell IsolationSingle Cell SuspensionALDH AssayColony Forming AssayFunctional CharacterizationTumorigenicityMetastasis

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