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Cancer Research
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研究结肠癌干细胞的球体三维模型
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
研究结肠癌干细胞的球体三维模型
DOI:
10.3791/61783-v
•
06:38 min
•
January 22, 2021
•
Maria Virginia Giolito
2
,
Léo Claret
2
,
Carla Frau
,
Michelina Plateroti
2
1
Département de la recherche
,
Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard
,
2
UMR-S1113 – IRFAC INSERM, Université de Strasbourg
Chapters
00:04
Introduction
01:05
Spheroid Culture Preparation
01:47
Spheroid Generation
03:24
Spheroid Harvest
04:30
Results: Representative Colon Cancer Stem Cell Spheroid Formation and Analysis
06:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了一个新型的,强大的,可重复的文化系统,以产生和生长三维球体从Caco2结肠腺癌细胞。研究结果为研究癌症干细胞生物学(包括化疗反应)的方法的恰当性提供了第一个概念证明。
Tags
3D Spheroid Model
Colon Cancer Stem Cells
Tumorigenicity
Cancer Stem Cell Analysis
High-throughput Drug Screening
Homogeneous Spheroid Culture
3D Culture Conditions
Caco-2 Cells
DMEM Basement Membrane Matrix Medium
Cell Counting
Cell Seeding
Centrifugation
Microscopic Observation
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