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JoVE Journal Cancer Research
En tredimensjonal modell av sfæroider for å studere kolonkreft stamceller
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En tredimensjonal modell av sfæroider for å studere kolonkreft stamceller

Article DOI: 10.3791/61783-v • 06:38 min • January 22nd, 2021
January 22nd, 2021 •
1,2, 1,2, 1, 1,2
1Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 - IRFAC INSERM, Université de Strasbourg

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Denne protokollen presenterer et nytt, robust og reproduserbart kultursystem for å generere og vokse tredimensjonale sfæroider fra Caco2 kolon adenokarsinomceller. Resultatene gir det første konseptbeviset for hensiktsmessigheten av denne tilnærmingen for å studere kreft stamcellebiologi, inkludert responsen på kjemoterapi.

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Kreftforskning Utgave 167 Caco2 celler Kreft stamceller Celleproliferasjon Tykktarmskreft Colonospheres Colonospheres vekst
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