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Journal / Bioengineering / Segmentering vækst af endotelceller i 6-brønd…
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JoVE Journal Bioengineering
Segmentering vækst af endotelceller i 6-brønd plader på en Orbital Shaker for mekanobiologiske undersøgelser
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Segmentering vækst af endotelceller i 6-brønd plader på en Orbital Shaker for mekanobiologiske undersøgelser

Article DOI: 10.3791/61817-v • 12:28 min • June 3rd, 2021
June 3rd, 2021 •
*1,2, *1, 1, 2,3,4, 1
1Department of Bioengineering, Imperial College London, 2Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), 3Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, 4Singapore Eye Research Institute, The Academia
* These authors contributed equally

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Denne protokol beskriver en belægning metode til at begrænse endotelcelle vækst til et bestemt område af en 6-brønds plade til forskydning stress ansøgning ved hjælp af orbital shaker model.

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Tilbagetrækning Problem 172 Segmentering Vækst Endotel Shear Stress Orbital Shaker Mekanobiologi Lav Magnitude Multidirectional Flow Høj Størrelse Uniaxial Flow PDMS Pluronic F-127 tværgående væg shear stress
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