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Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

DOI:
10.3791/61832-v

•

07:40 min

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January 12, 2022

•

, , ,
1UCL Institute of Ophthalmology,University College London

Chapters

  • 00:05Introduction
  • 00:57IRBP1-20-Complete Freund s Adjuvant (CFA) Emulsion Preparation and Injection
  • 03:00Mouse Fundus Examination and Fluorescence Angiography
  • 04:40Clinical Disease and Histological Scoring
  • 06:06Results: Representative Fundoscopic, Histologic, and Fluorescence Imaging
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

In this report we present a protocol that allows the investigator to generate a mouse model of intraocular uveitis. More commonly referred to as experimental autoimmune uveitis (EAU), this established model captures many aspects of human disease. Herein, we will describe how to induce and monitor disease progression using several readouts.

Tags

Experimental Autoimmune UveitisIntraocular Inflammatory Mouse ModelIRBP 1-20Complete Freund’s AdjuvantBordetella Pertussis ToxinDisease InductionNon-invasive Endoscopic TechniquesDisease ProgressionClinical Disease ScoringTropicamidePhenylephrine

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