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Biology

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
 

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Article DOI: 10.3791/61855-v
November 3rd, 2020

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Summary November 3rd, 2020

Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.

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