Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Kanut Laoharawee; Matthew J. Johnson; Walker S. Lahr; Joseph J. Peterson; Beau R. Webber; Branden S. Moriarity

doi:10.3791/61855

Journal

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Biology

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

JoVE Journal
Biology

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JoVE Journal Biology

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

6,223 Views

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08:20 min

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November 03, 2020

DOI:

10.3791/61855-v

DOI:

10.3791/61855-v

•

08:20 min

November 03, 2020

•

6207 Views

Kanut Laoharawee^1,2,3, Matthew J. Johnson^1,2,3, Walker S. Lahr^1,2,3, Joseph J. Peterson^1,2,3, Beau R. Webber^1,2,3, Branden S. Moriarity^1,2,3

¹Department of Pediatrics,University of Minnesota ²Center for Genomic Engineering,University of Minnesota ³Masonic Cancer Center,University of Minnesota

Summary

Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.