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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
JoVE Journal
Biology
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JoVE Journal Biology
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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08:20 min

November 03, 2020

DOI:

08:20 min
November 03, 2020

6207 Views
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Summary

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Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.

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