Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Genomteknikk av primære humane B-celler ved hjelp …
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Genomteknikk av primære humane B-celler ved hjelp av CRISPR/Cas9
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Journal (Biology)
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
Journal (Biology)
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
 
Click here for the English version

Genomteknikk av primære humane B-celler ved hjelp av CRISPR/Cas9

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her tilbyr vi en detaljert, trinnvis protokoll for CRISPR/Cas9-basert genomteknikk av primære humane B-celler for genutslag (KO) og knock-in (KI) for å studere biologiske funksjoner av gener i B-celler og utvikling av B-celleterapeutikk.

Tags

Biologi Utgave 165 CRISPR/Cas9 genomteknikk rekombinant AAV genredigering primære humane B-celler
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter