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Journal / Biochemistry / Laserfreies Hydroxylradikalprotein-Footprinting…
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Biochemistry

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JoVE Journal Biochemistry
Laserfreies Hydroxylradikalprotein-Footprinting zur Durchführung einer Strukturanalyse höherer Ordnung von Proteinen
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Laserfreies Hydroxylradikalprotein-Footprinting zur Durchführung einer Strukturanalyse höherer Ordnung von Proteinen

Article DOI: 10.3791/61861-v • 09:59 min • June 4th, 2021
June 4th, 2021 •
1, 1, 1,2,3, 2
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, 3Department of Chemistry and Biochemistry, University of Mississippi

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Dieses Protokoll stellt eine Methode zur Verwendung von Inline-Radikaldosimetrie und einer Plasmalichtquelle vor, um Flash-Oxidationsprotein-Footprinting durchzuführen. Diese Methode ersetzt den gefährlichen UV-Laser, um die Reproduzierbarkeit der schnellen photochemischen Oxidation von Proteinstudien zu vereinfachen und zu verbessern.

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Biochemistry Biopharmaceutical Biosimilar Higher Order Structure Fast Photochemical Oxidation of Proteins (FPOP) Hydroxyl Radical Protein Footprinting (HRPF) Epitope Mapping Receptor-Drug Interaction Protein-Protein Interaction
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