A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion

The mouse model established with this protocol can be utilized to investigate the pathophysiology of ischemic retinopathies and for in vivo drug screening. The current method could reduce reliance on various surgical instruments and shorten operation time to induce retinal ischemia. Pathogenesis of vascular diseases, such as diabetic retinopathy, occlusion of retinal veins or arteries and ocular ischemic syndrome can be understood.

Demonstrating the procedure will be Deokho Lee, our PhD student from my laboratory. Yukihiro Miwa, DVM, PhD, and assistant professor from my laboratory will help to demonstrate the procedure. Autoclave all the surgical instruments and keep them in 70%ethyl alcohol prior to the surgical procedure.