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A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

DOI:
10.3791/61867-v

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09:17 min

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January 05, 2021

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, , , , , , ,
1Institute of Molecular Biology and Pathology,National Research Council of Italy (CNR), 2Department of Molecular Medicine,University of Rome “Sapienza”, 3Center for Life Nano Science,Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed

Chapters

  • 00:04Introduction
  • 00:57Intracellular Staining
  • 02:41Data Analysis
  • 07:16Results: Cell Cycle Analysis of BM Cells and Antigen-Specific CD8 T Cells form LNs and Spleen
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.

Tags

Keywords: DNAKi67Flow CytometryCell CycleCD8 T CellsVaccinationImmune DynamicsPermeabilization BufferHoechstAntigen-Specific

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