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Journal / Immunology and Infection / A DNA/Ki67-Based Flow Cytometry Assay for Cell…
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Immunology and Infection

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JoVE Journal Immunology and Infection
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A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Article DOI: 10.3791/61867-v • 09:17 min • January 5th, 2021
January 5th, 2021 •
*1,2, *1,2, 3, 4, 5, 5, 2,6, 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
* These authors contributed equally

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Summary
Automatic Translation

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.

Tags

DNA/Ki67-based Flow Cytometry Assay Cell Cycle Analysis Antigen-specific CD8 T Cells Vaccinated Mice T Cell Response Phenotypic Snapshot Proliferating CD8 T Cells In Vivo Drug Treatment Cell Transfer Immune Dynamics Vaccination Response Infections Autoimmune Diseases Cancer Immunotherapy Permeabilization Wash Buffer Monoclonal Antibody Ki67 FITC Sample Preparation
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