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Journal / Immunology and Infection / En DNA/Ki67-baseret flowcytometrianalyse til…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
En DNA/Ki67-baseret flowcytometrianalyse til cellecyklusanalyse af antigenspecifikke CD8 T-celler i vaccinerede mus
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En DNA/Ki67-baseret flowcytometrianalyse til cellecyklusanalyse af antigenspecifikke CD8 T-celler i vaccinerede mus

Article DOI: 10.3791/61867-v • 09:17 min • January 5th, 2021
January 5th, 2021 •
*1,2, *1,2, 3, 4, 5, 5, 2,6, 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
* These authors contributed equally

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Kloningsudvidelse er et centralt element i antigenspecifik T-cellerespons. Imidlertid er cellecyklussen af antigenoptalende T-celler blevet dårligt undersøgt, dels på grund af tekniske begrænsninger. Vi beskriver en flow cytometrisk metode til at analysere clonally ekspanderende antigen-specifikke CD8 T-celler i milt og lymfeknuder af vaccinerede mus.

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Immunologi og infektion Problem 167 antigen-specifikke CD8 T-celler cellecyklus Ki67 DNA-farvestof flowcytometri milt lymfeknuder mus
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