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Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

DOI:
10.3791/61868-v

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05:52 min

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November 25, 2020

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, , , , ,
1Institute for Ophthalmic Research,University of Tübingen

Chapters

  • 00:04Introduction
  • 01:07Explant Preparation
  • 03:46Results: Analysis of Cell Types Found in Retinal Explant
  • 04:49Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes organotypic explants of mouse neuroretina, cultivated together with its retinal pigment epithelium (RPE), in R16 defined medium, free of serum and antibiotics. This method is relatively simple to perform, less expensive, and time-consuming when compared to in vivo experiments, and can be adapted to numerous experimental applications.

Tags

Keywords: Organotypic Retina ExplantSerum-free CultureRetinal Pigment EpitheliumRetinitis PigmentosaDiabetic RetinopathyIn Vitro Retinal ModelPre-clinical Treatment Development

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