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JoVE Journal
Bioengineering

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
 

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Article DOI: 10.3791/61879-v 10:37 min March 14th, 2021
March 14th, 2021

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Summary

Here, we describe an easy-to-use methodology to generate 3D self-assembled cardiac microtissue arrays composed of pre-differentiated human-induced pluripotent stem cell-derived cardiomyocytes, cardiac fibroblasts, and endothelial cells. This user-friendly and low cell requiring technique to generate cardiac microtissues can be implemented for disease modeling and early stages of drug development.

Tags

Keywords: 3D Cardiac Microtissue Human IPSC-derived Cardiomyocytes Cardiac Fibroblasts Endothelial Cells Agarose Micromold Cardiac Spheroids Disease Modeling Drug Testing Contractile Function Imaging Cell Suspension Cell Seeding Medium Change
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