Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Dilip Thomas; Hyeonyu Kim; Nicole Lopez; Joseph C. Wu

doi:10.3791/61879

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

6,408 Views

•

10:37 min

•

March 14, 2021

DOI:

10.3791/61879-v

DOI:

10.3791/61879-v

•

10:37 min

March 14, 2021

•

6369 Views

Dilip Thomas¹, Hyeonyu Kim¹, Nicole Lopez¹, Joseph C. Wu^1,2,3

¹Stanford Cardiovascular Institute,Stanford University School of Medicine ²Department of Medicine, Division of Cardiovascular Medicine,Stanford University School of Medicine ³Department of Radiology,Stanford University School of Medicine

Summary

Here, we describe an easy-to-use methodology to generate 3D self-assembled cardiac microtissue arrays composed of pre-differentiated human-induced pluripotent stem cell-derived cardiomyocytes, cardiac fibroblasts, and endothelial cells. This user-friendly and low cell requiring technique to generate cardiac microtissues can be implemented for disease modeling and early stages of drug development.