Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Time-Resolved Fluorescence Imaging and Analysis…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
Journal (Biology)
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
Journal (Biology)
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
 

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Article DOI: 10.3791/61902-v • 07:42 min • January 30th, 2021
January 30th, 2021 •
1, 1, 1,2
1Bioengineering Department, Temple University, 2Cancer Biology Program, Fox Chase Cancer Center

Chapters

Summary
Automatic Translation

Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

Tags

Time-resolved Fluorescence Imaging Cancer Cell Invasion 3D Spheroid Model Mechanisms Extracellular Matrix Spheroid Imaging Device Spheroid Invasion Assay Mammary Carcinoma Solid Tumor 3D Printing Base Polymer Cross-linker PDMS Solution Vacuum Chamber Glass Plates Binder Clips Mold
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter