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3D球形模型中癌细胞入侵的时限解荧光成像和分析

JoVE Journal
Biology

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JoVE Journal Biology

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

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3D球形模型中癌细胞入侵的时限解荧光成像和分析

DOI:

10.3791/61902-v

•

07:42 min

•

January 30, 2021

•

Louisiane Perrin, Theodore Tucker, Bojana Gligorijevic²

¹Bioengineering Department,Temple University, ²Cancer Biology Program,Fox Chase Cancer Center

Chapters

00:05Introduction
00:39Spheroid Imaging Device (SID) Fabrication
04:51Spheroid Collagen Embedding
06:06Results: Representative Spheroid Imaging
07:06Conclusion

Summary

Automatic Translation

这里介绍了一个制造球形成像设备的协议。该设备可对癌细胞球体进行动态或纵向荧光成像。该协议还提供了一个简单的图像处理程序，用于分析癌细胞入侵。

Tags

3D Spheroid Model Cancer Cell Invasion Time-resolved Fluorescence Imaging Extracellular Matrix PDMS 3D Printing Spacer Glass Plates Vacuum Chamber Biopsy Punch Mammary Carcinoma

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