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In vivo Evaluation of Mucociliary Clearance in Mice
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In vivo Evaluation of Mucociliary Clearance in Mice

In vivo Evaluation of Mucociliary Clearance in Mice

DOI:
10.3791/61929-v

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06:35 min

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December 18, 2020

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,
1Department of Developmental Biology,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 00:39Mouse Intubation and Instillation
  • 02:06Single Photon Emission Computed Tomography and Computed Tomography (SPECT/CT) Imaging
  • 03:10Analysis
  • 04:48Results: Representative Mouse Mucociliary Clearance (MCC) Evaluation
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

In this publication, we describe protocols for assessing airway mucociliary clearance (MCC) in mice in vivo utilizing dual-modality radionuclide imaging. This protocol is designed for a single photon emission computed tomography (SPECT) and computed tomography (CT) acquisition protocol using mouse whole body (MWB) collimators in a dual SPECT/CT system.

Tags

Keywords: Mucociliary ClearanceIn Vivo EvaluationMiceRadionuclidesSPECT/CT ImagingAnesthesiaIntubationTechnetium Sulfur Colloid3D ReconstructionImage Analysis

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