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Journal / Bioengineering / Padronização simples, acessível e modular de…
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Bioengineering

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JoVE Journal Bioengineering
Padronização simples, acessível e modular de células usando DNA
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Padronização simples, acessível e modular de células usando DNA

Article DOI: 10.3791/61937-v • 08:59 min • February 24th, 2021
February 24th, 2021 •
1, 2, 1, 3, 4,5,6, 7,8, 9, 2,6,10
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry, University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 5California Institute for Quantitative Biosciences, University of California San Francisco, 6Chan Zuckerberg Biohub, University of California San Francisco, 7Department of Chemical & Biomolecular Engineering, University of California Berkeley, 8Helen Wills Neuroscience Institute, University of California Berkeley, 9Department of Mechanical Engineering, University of California Berkeley, 10Center for Cellular Construction, University of California San Francisco

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Aqui apresentamos um protocolo para células de micropattern em resolução unicelular usando a adesão programada pelo DNA. Este protocolo usa uma plataforma de fotolitografia benchtop para criar padrões de oligonucleotídeos de DNA em um slide de vidro e, em seguida, rotula membranas celulares com oligonucleotídeos complementares disponíveis comercialmente. A hibridização dos oligos resulta em adesão celularprogramada.

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Bioengenharia Edição 168 micropatterning engenharia de tecidos bioengenharia cultura celular 3D fotolithografia microfabricação biologia celular
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