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A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

DOI:
10.3791/61981-v

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11:24 min

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June 30, 2021

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1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Department of Genetics,Albert Einstein College of Medicine

Chapters

  • 00:00Introduction
  • 00:21Zebrafish Embryo Collection
  • 01:50Tools Preparation
  • 03:33Media Recipes
  • 05:183-D Tail Explants
  • 08:42Confocal Microscopy with 3-D Explants
  • 10:30Representative Results
  • 10:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

Tags

Keywords: 3-D Tail ExplantZebrafishVertebrate SegmentationSomite DevelopmentPresomitic MesodermEmbryo TransparencyTime-lapse ImagingDissection TechniquesVertebrate Patterning

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