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Journal / Genetics / CRISPR/Cas9 使用dpy-10联合 CRISPR…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 使用dpy-10联合 CRISPR 筛选标记和组装的核糖核蛋白复合物编辑C. elegans rbm-3.2基因。
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CRISPR/Cas9 使用dpy-10联合 CRISPR 筛选标记和组装的核糖核蛋白复合物编辑C. elegans rbm-3.2基因。

Article DOI: 10.3791/62001-v • 07:46 min • December 11th, 2020
December 11th, 2020 •
1, *1, *1, *1, 1
1Department of Chemistry and Biochemistry, The University of Tulsa
* These authors contributed equally

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Summary
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此协议的目标是使用组装的核糖核蛋白复合物和dpy-10共同 CRISPR 标记进行无缝的 CRISPR/Cas9 编辑，以便进行筛选。 此协议可用于在C. elegans中进行各种基因修改，包括插入、删除、基因替换和 codon 替代。

Tags

遗传学， 第 166 期， CRISPR/Cas9， C. 电子甘，核糖核蛋白复合物， 基因组工程， rbm-3.2， 基因编辑， 微注射， CRISPR 筛选
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