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Journal / Genetics / CRISPR/Cas9 Redigering af C. elegans rbm-3.2…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 Redigering af C. elegans rbm-3.2 Genet ved hjælp af dpy-10 Co-CRISPR Screening Marker og Samlet Ribonucleoprotein Complexes.
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CRISPR/Cas9 Redigering af C. elegans rbm-3.2 Genet ved hjælp af dpy-10 Co-CRISPR Screening Marker og Samlet Ribonucleoprotein Complexes.

Article DOI: 10.3791/62001-v • 07:46 min • December 11th, 2020
December 11th, 2020 •
1, *1, *1, *1, 1
1Department of Chemistry and Biochemistry, The University of Tulsa
* These authors contributed equally

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Målet med denne protokol er at muliggøre problemfri CRISPR /Cas9 redigering af C. elegans genom ved hjælp af samlede ribonucleoprotein komplekser og dpy-10 co-CRISPR markør til screening. Denne protokol kan bruges til at foretage en række genetiske modifikationer i C. elegans herunder indsættelser, sletninger, generstatninger og codon substitutioner.

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Genetik Udgave 166 CRISPR/Cas9 C. elegans ribonucleoprotein komplekser genom engineering rbm-3.2 genredigering microinjection CRISPR screening
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