Genetics
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CRISPR/Cas9 C. elegans rbm-3.2 Geninin dpy-10 Co-CRISPR Tarama İşaretçisi ve Monte Edilmiş Ribonikleoprotein Kompleksleri kullanılarak düzenlenmesi.
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Summary December 11th, 2020
Please note that all translations are automatically generated.
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Bu protokolün amacı, C. elegans genomunun monte edilmiş ribonikleoprotein kompleksleri ve tarama için dpy-10 co-CRISPR işaretleyicisi kullanılarak sorunsuz CRISPR/Cas9 düzenlemesini sağlamaktır. Bu protokol, C. elegans'ta eklemeler, silmeler, gen değiştirmeleri ve kodon ikameleri de dahil olmak üzere çeşitli genetik değişiklikler yapmak için kullanılabilir.
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