JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
隔离和成像现场, 通过颤音分割老鼠肾佩尔维斯的精致起搏器区域
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
Please note that all translations are automatically generated.
Click here for the English version.
隔离和成像现场, 通过颤音分割老鼠肾佩尔维斯的精致起搏器区域
DOI:
10.3791/62040-v
•
07:38 min
•
April 30, 2021
•
Nathan Grainger
2
,
Kenton M. Sanders
,
Bernard T. Drumm
3
1
Department of Physiology & Cell Biology
,
University of Nevada, Reno School of Medicine
,
2
Department of Physiology & Membrane Biology
,
University of California School of Medicine
,
3
Department of Life & Health Sciences
,
Dundalk Institute of Technology
Chapters
00:05
Introduction
00:44
Preparation and Dissection of Kidney and Calibration of Vibratome Instrument
02:32
Vibratome Sectioning and Kidney Slice Ca
2+
Image Acquisition
04:06
Kidney Slice Ca
2+
Image Acquisition
05:14
Results: Imaging of Spontaneous Calcium Ion Transient in Vibratome Sections of Whole Kidneys
07:01
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
此协议的目标是使用振动切除隔离小鼠肾骨盆的完整心脏起搏器区域。然后,这些部分可用于原位 Ca
2+
成像,以阐明心脏起搏器细胞和振动切片中其他间质细胞的 Ca
2+
瞬态特性。
Tags
Pacemaker Regions
Renal Pelvis
Vibratome Sectioning
Intact Preparation
Smooth Muscle
Kidney
Ureter
Calcium Ion Imaging
Dissection
Razor Blade
KRB Solution
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
现场共聚焦显微镜对斑马鱼的胚胎脑成像
活体
成像和治疗卵巢癌的原位小鼠模型
小鼠胚胎皮肤和Live成像黑素细胞迁移的
体外
培养
维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养
在
造血干细胞和祖细胞的成年小鼠颅骨骨髓
体内
四维跟踪
在肾小管上皮细胞胞浆钙的测量流式细胞仪
具体的基因组区域及相关分子鉴定enChIP隔离
从冷冻芯的外层部分外源物质的去除探讨古代生物群落里面窝藏
肾水动力肾盂注射非病毒蛋白在肾脏的表达
单个细胞分辨率三维成像的有机体
Read Article